CC-4782 rbcL-G171D mt+ (10-6C) (non-pf2 progeny used for genetic analysis)
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Phenotype: requires acetate, sensitive to light
Following 5-fluorodeoxyuridine treatment and ethyl-methanesulfonate mutagenesis of wild-type 2137 mt+ cells, colonies were screened for light-sensitive, acetate-requiring phenotypes (Spreitzer and Mets 1981). Mutant 10-6C was found to arise from a G171D substitution (GGT-GAT) in the chloroplast-encoded large subunit of Rubisco (Dron et al. 1983), which causes a decrease in Rubisco activities and alters the large-subunit isoelectric point (Spreitzer and Mets 1980; Spreitzer et al. 1982). Selection for photosynthesis-competent revertants yielded only true revertants (Spreitzer et al. 1982, 1985). This strain was recovered from a cross between 10-6C mt+ (CC-1815) and pf2 mt-, and has been maintained with acetate medium in darkness since 1980.
Dron M, Rahire M, Rochaix JD, Mets L (1983) First DNA sequence of a chloroplast mutation: A missense alteration in the ribulosebisphosphate carboxylase large subunit gene. Plasmid 9:321-324
Spreitzer RJ, Jordan DB, Ogren WL (1982) Biochemical and genetic analysis of an RuBP carboxylase/oxygenase mutant and revertants of Chlamydomonas reinhardtii. FEBS Lett 148:117-121
Spreitzer RJ, Rahire M, Rochaix JD (1985) True reversion of a mutation in the chloroplast gene encoding the large subunit of ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas. Curr Genet 9:229-231
Spreitzer RJ, Mets L (1980) Non-mendelian mutation affecting ribulose-1,5-bisphosphate carboxylase structure and activity. Nature 285:114-115
Spreitzer RJ, Mets L (1981) Photosynthesis-deficient mutants of Chlamydomonas with associated light-sensitive phenotypes. Plant Physiol 67:565-569