CC-4785 rbcL-W66Amber mt+ (18-7G) (non-pf2 progeny used for genetic analysis)
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Phenotype: requires acetate, sensitive to light
The 18-7G chloroplast mutant was recovered by screening a collection of acetate-requiring mutants for lack of complementation with a known Rubisco rbcL mutant (Spreitzer and Ogren 1983). It results from an rbcL nonsense mutation that changes the codon for Trp-66 (TGG) to amber (TAG) (Spreitzer et al. 1985). Selection for photosynthesis-competent revertants yielded heteroplasmic strains that result from chloroplast informational (tRNA) suppression (Spreitzer and Chastain 1987; Yu and Spreitzer 1992). This strain was recovered from a cross between the original 18-7G mt+ (CC-2653) and pf2 mt-, and has been used as a host for chloroplast rbcL transformation. It has been maintained with acetate medium in darkness since its isolation.
Spreitzer RJ, Chastain CJ (1987) Heteroplasmic suppression of an amber mutation in the Chlamydomonas chloroplast gene that encodes the large subunit of ribulosebisphosphate carboxylase/oxygenase. Curr Genet 11:611-616
Spreitzer RJ, Goldschmidt-Clermont M, Rahire M, Rochaix JD (1985) Nonsense mutations in the Chlamydomonas chloroplast gene that codes for the large subunit of ribulosebisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 82:5460-5464
Spreitzer RJ, Ogren WL (1983) Rapid recovery of chloroplast mutations affecting ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 80:6293-6297
Yu W, Spreitzer RJ (1992) Chloroplast heteroplasmicity is stabilized by an amber-suppressor tryptophan tRNA(CUA). Proc Natl Acad Sci USA 89:3904-3907