CC-4787 rbcL-R217S mt+ (28-7J) (non-pf2 progeny used for genetic analysis)
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Phenotype: requires acetate, sensitive to light
The 28-7J chloroplast mutant was recovered by screening a collection of acetate-requiring mutants for lack of complementation with a known Rubisco rbcL mutant. The mutant was crossed with pf2 mt- to confirm uniparental inheritance, and this mt+ progeny clone was analyzed. Mutant 28-7J results from an R217S substitution (CGT-AGT) in the chloroplast-encoded large subunit of Rubisco, which blocks Rubisco holoenzyme assembly (Thow et al. 1994). Selection for photosynthesis-competent revertants yielded true revertants, intragenic suppressors (see R14-A), and heteroplasmic suppressors (Thow et al. 1994) that likely arise from heteroplasmic informational (tRNA) suppression (Yu and Spreitzer 1992). This strain has been maintained with acetate medium in darkness since its isolation.
Thow G, Zhu G, Spreitzer RJ (1994) Complementing substitutions within loop regions 2 and 3 of the alpha/beta-barrel active site influence the CO2/O2 specificity of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase. Biochemistry 33:5109-5114
Yu W, Spreitzer RJ (1992) Chloroplast heteroplasmicity is stabilized by an amber-suppressor tryptophan tRNA(CUA). Proc Natl Acad Sci USA 89:3904-3907