CC-4789 rbcL-R217S/A242V pf2 mt- (R14-A revertant of 28-7J)
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Phenotype: requires acetate at 35 °C, temperature-conditional
R14-A was recovered as a photosynthesis-competent revertant of rbcL-R217S mt+ (28-7J). It results from an intragenic-suppressor mutation that causes an A242V substitution (GCT-GTT) in the Rubisco large subunit, and the double-mutant enzyme has decreases in Rubisco CO2/O2 specificity, holoenzyme stability, and large-subunit isoelectric point (Thow et al. 1994). The mutant can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. This strain was recovered from a cross between rbcL-R217S/A242V mt+ and pf2 mt-. It has been maintained with acetate medium in darkness since its isolation.
Thow G, Zhu G, Spreitzer RJ (1994) Complementing substitutions within loop regions 2 and 3 of the alpha/beta-barrel active site influence the CO2/O2 specificity of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase. Biochemistry 33:5109-5114