CC-4790 rbcL-A242V mt+ (S14-A suppressor of 28-7J)
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Using standard methods of directed mutagenesis and chloroplast-gene transformation of rbcL∆-25B1 mt+ (CC-4700) (Zhu and Spreitzer 1996), Genhai Zhu in Spreitzer’s group created an A242V substitution (GCT-GTT) in the Rubisco large subunit. This is the intragenic-suppressor substitution that complements the R217S substitution in photosynthesis-competent revertant R14-A (rbcL-R217S/A242V) (Thow et al. 1994). Preliminary analysis indicated that the A242V substitution has little or no effect on Rubisco catalysis or holoenzyme stability (Zhu and Spreitzer, unpublished). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Thow G, Zhu G, Spreitzer RJ (1994) Complementing substitutions within loop regions 2 and 3 of the alpha/beta-barrel active site influence the CO2/O2 specificity of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase. Biochemistry 33:5109-5114
Zhu G, Spreitzer RJ (1996) Directed mutagenesis of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase: Loop-6 substitutions complement for structural stability but decrease catalytic efficiency. J Biol Chem 271:18494-18498