CC-4793 rbcL-G54V mt+ (R95-1C revertant of 31-4E)
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
R95-1C was recovered as a photosynthesis-competent revertant of rbcL-G54D mt+ (31-4E). It results from a pseudoreversion mutation that causes an D54V substitution (GAT-GTT), which results in a G54V substitution (GGT-GTT) in the Rubisco large subunit (Spreitzer et al. 1995). The G54V substitution causes a decrease in Rubisco CO2/O2 specificity and holoenzyme stability. This strain has been maintained with acetate medium in darkness since its isolation.
Spreitzer RJ, Thow G, Zhu G (1995) Pseudoreversion substitution at large-subunit residue 54 influences the CO2/O2 specificity of chloroplast ribulosebisphosphate carboxylase/oxygenase. Plant Physiol 109:681-686