CC-4794 rbcL-G54A mt+ (R95-10A revertant of 31-4E)
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
R95-10A was recovered as a photosynthesis-competent revertant of rbcL-G54D mt+ (31-4E). It results from a pseudoreversion mutation that causes an D54A substitution (GAT-GCT), which results in a G54A substitution (GGT-GCT) in the Rubisco large subunit (Spreitzer et al. 1995). The G54A substitution causes a decrease in Rubisco holoenzyme stability. This strain has been maintained with acetate medium in darkness since its isolation.
Spreitzer RJ, Thow G, Zhu G (1995) Pseudoreversion substitution at large-subunit residue 54 influences the CO2/O2 specificity of chloroplast ribulosebisphosphate carboxylase/oxygenase. Plant Physiol 109:681-686