CC-4797 rbcL-M42V/C53A mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Directed mutagenesis and chloroplast transformation of rbcL-W66Amber mt+ (Spreitzer et al. 1985) were used to create M42V (ATG-GTA) and C53A (TGT-GCT) substitutions in the Rubisco large subunit, which cause a decrease in Rubisco CO2/O2 specificity (Du et al. 2003). This is the original double-mutant strain. It was created to investigate phylogenetic differences near large-subunit residue Gly-54 (see rbcL-G54D) (Spreitzer et al. 1995). The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Du YC, Peddi SR, Spreitzer RJ (2003) Assessment of structural and functional divergence far from the large subunit active site of ribulose-1,5-bisphosphate carboxylase/oxygenase. J Biol Chem 278:49401-49405
Spreitzer RJ, Goldschmidt-Clermont M, Rahire M, Rochaix JD (1985) Nonsense mutations in the Chlamydomonas chloroplast gene that codes for the large subunit of ribulosebisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 82:5460-5464
Spreitzer RJ, Thow G, Zhu G (1995) Pseudoreversion substitution at large-subunit residue 54 influences the CO2/O2 specificity of chloroplast ribulosebisphosphate carboxylase/oxygenase. Plant Physiol 109:681-686