CC-4798 rbcL-A99C mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Directed mutagenesis and chloroplast transformation of rbcL∆-25B1 mt+ (CC-4700) were used to create an A99C substitution in the Rubisco large subunit. This mutant was created to investigate phylogenetic differences near large-subunit residue Gly-54 (see rbcL-G54D) (Spreitzer et al. 1995; Du et al. 2003). The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
William Zerges reported CC-4798 should have A99C but it is wild type, at least in the 5 portions where he sequenced, including that position. (personal communication, 12/8/20)
Du YC, Peddi SR, Spreitzer RJ (2003) Assessment of structural and functional divergence far from the large subunit active site of ribulose-1,5-bisphosphate carboxylase/oxygenase. J Biol Chem 278:49401-49405
Spreitzer RJ, Thow G, Zhu G (1995) Pseudoreversion substitution at large-subunit residue 54 influences the CO2/O2 specificity of chloroplast ribulosebisphosphate carboxylase/oxygenase. Plant Physiol 109:681-686