CC-4799 rbcL-C53A/A99C mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Directed mutagenesis and chloroplast transformation of rbcL∆-25B1 mt+ (CC-4700) were used to create C53A and A99C substitutions in the Rubisco large subunit. This double mutant was created to investigate phylogenetic differences near large-subunit residue Gly-54 (see rbcL-G54D) (Spreitzer et al. 1995; Du et al. 2003). The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Du YC, Peddi SR, Spreitzer RJ (2003) Assessment of structural and functional divergence far from the large subunit active site of ribulose-1,5-bisphosphate carboxylase/oxygenase. J Biol Chem 278:49401-49405
Spreitzer RJ, Thow G, Zhu G (1995) Pseudoreversion substitution at large-subunit residue 54 influences the CO2/O2 specificity of chloroplast ribulosebisphosphate carboxylase/oxygenase. Plant Physiol 109:681-686