CC-4805 rbcL-T342I mt+ (S40-9D suppressor of 45-3B)
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Using standard methods of directed mutagenesis and chloroplast-gene transformation of rbcL∆-MX3312 mt+ (CC-4696), a T342I substitution (ACT-ATT) was created in the Rubisco large subunit (Karkehabadi et al. 2007). This is the intragenic-suppressor substitution that complements the V331A substitution in photosynthesis-competent revertant R40-9D (rbcL-V331A/T342I) (Chen and Spreitzer 1989; Chen et al. 1991). The T342I substitution alone decreases the CO2/O2 specificity of Rubisco, and the x-ray crystal structure of the T342I mutant Rubisco has been solved (Karkehabadi et al. 2007). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Chen Z, Spreitzer RJ (1989) Chloroplast intragenic suppression enhances the low CO2/O2 specificity of mutant ribulosebisphosphate carboxylase/oxygenase. J Biol Chem 264:3051-3053
Chen Z, Yu W, Lee JH, Diao R, Spreitzer RJ (1991) Complementing amino-acid substitutions within loop 6 of the alpha/beta-barrel active site influence the CO2/O2 specificity of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase. Biochemistry 30:8846-8850
Karkehabadi S, Satagopan S, Taylor TC, Spreitzer RJ, Andersson I (2007) Structural analysis of altered large-subunit loop-6/carboxy-terminus interactions that influence catalytic efficiency and CO2/O2 specificity of ribulose-1,5-bisphosphate carboxylase/oxygenase. Biochemistry 46:11080-11089