CC-4830 rbcL-Y190L mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Directed mutagenesis and chloroplast transformation of rbcL∆-MX3312 mt+ (CC-4696) were used to create a Y190L substitution in the Rubisco large subunit, which causes small decreases in Rubisco carboxylation and holoenzyme stability (Esquivel and Spreitzer, unpublished). This mutant strain was created to investigate the molecular basis for crosslinking between Rubisco large and small subunits (Ferreira et al. 1996). Other nearby Tyr residues have been studied (Esquivel et al. 2006). The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Esquivel MG, Pinto TS, Marin-Navarro J, Moreno J (2006) Substitution of tyrosine residues at the aromatic cluster around the betaA-betaB loop of Rubisco small subunit affects the structural stability of the enzyme and the in vivo degradation under stress conditions. Biochemistry 45:5745-5753
Ferreira RM, Franco E, Teixeira AR (1996) Covalent dimerization of ribulose bisphosphate carboxylase subunits by UV radiation. Biochem 318:227-234