CC-4831 rbcL-Y226L mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Directed mutagenesis and chloroplast transformation of rbcL-W66Amber mt+ were used to create a Y226L substitution (TAC-TTA) in the Rubisco large subunit, which causes decreases in Rubisco carboxylation and holoenzyme stability (Esquivel et al. 2006). This mutant strain was created to investigate Rubisco degradation. Other nearby Tyr residues have been studied (Esquivel et al. 2006). The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Esquivel MG, Pinto TS, Marin-Navarro J, Moreno J (2006) Substitution of tyrosine residues at the aromatic cluster around the betaA-betaB loop of Rubisco small subunit affects the structural stability of the enzyme and the in vivo degradation under stress conditions. Biochemistry 45:5745-5753