CC-4837 rbcL-A222T mt+ (S96-8E suppressor of 68-4PP)
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
Using standard methods of directed mutagenesis and chloroplast-gene transformation of rbcL∆-25B1 mt+ (CC-4700), an A222T substitution (GCT-ACA) was created in the Rubisco large subunit (Du and Spreitzer 2000). This is the intragenic-suppressor substitution that complements the L290F substitution in photosynthesis-competent revertant R96-8E (rbcL-L290F/A222T) (Hong and Spreitzer 1997). The A222T substitution alone improves the thermal stability of wild-type Rubisco (Du and Spreitzer 2000). The x-ray crystal structure of L290F/A222T double-mutant Rubisco has been solved (Karkehabadi et al. 2005). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Du YC, Spreitzer RJ (2000) Suppressor mutations in the chloroplast-encoded large subunit improve the thermal stability of wild-type ribulose-1,5-bisphosphate carboxylase/oxygenase. J Biol Chem 275:19844-19847
Hong S, Spreitzer RJ (1997) Complementing substitutions at the bottom of the barrel influence catalysis and stability of ribulose-bisphosphate carboxylase/oxygenase. J Biol Chem 272:11114-11117
Karkehabadi S, Taylor TC, Spreitzer RJ, Andersson I (2005) Altered intersubunit interactions in crystal structures of catalytically-compromised ribulose-1,5-bisphosphate carboxylase/oxygenase. Biochemistry 44:113-120