CC-4847 rbcL-V221C/V235I/C256F/K258R/I265V mt+ (Penta)
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
Using a plasmid containing the rbcL-C256F/K258R/I265V gene (Du et al. 2003), directed mutagenesis and chloroplast transformation of rbcL∆-MX3312 mt+ (CC-4696) were used to create V221C (GTT-TGT), V235I (GTT-ATT), C256F (TGT-TTC), K258R (AAA-CGT), and I265V (ATT-GTA) substitutions in the Rubisco large subunit (Spreitzer et al. 2005). The addition of the V221C and V235I substitutions increases the CO2/O2 specificity of rbcL-C256F/K258R/I265V Rubisco (Du et al. 2003; Spreitzer et al. 2005). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Du YC, Peddi SR, Spreitzer RJ (2003) Assessment of structural and functional divergence far from the large subunit active site of ribulose-1,5-bisphosphate carboxylase/oxygenase. J Biol Chem 278:49401-49405
Spreitzer RJ, Peddi SR, Satagopan S (2005) Phylogenetic engineering at an interface between large and small subunits imparts land-plant kinetic properties to algal Rubisco. Proc Natl Acad Sci USA 102:17225-17230