CC-4848 rbcL-Penta/rbcS1-ABSO mt+ (penta/ABSO)
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
To create the penta/ABSO mutant, rbcL-V221C/V235I/C256F/K258R/I265V mt+ (Penta) was crossed with rbcS∆-CAL005.01.13 mt- (CC-4693), and an mt+ acetate-requiring progeny clone was recovered (Spreitzer et al. 2005). That strain was then transformed with an rbcS1-ABSO plasmid, which encodes a Rubisco small subunit containing the spinach betaA-betaB loop (Karkehabadi et al. 2005). Penta/ABSO Rubisco has increased CO2/O2 specificity and other kinetic properties similar to plant Rubisco enzymes (Spreitzer et al. 2005). This is the original mutant strain. It has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco expression.
Karkehabadi S, Peddi SR, Anwaruzzaman M, Taylor TC, Cederlund A, Genkov T, Andersson I, Spreitzer RJ (2005) Chimeric small subunits influence catalysis without causing global conformational changes in the crystal structure of ribulose-1,5-bisphosphate carboxylase/oxygenase. Biochemistry 44:9851-9861
Spreitzer RJ, Peddi SR, Satagopan S (2005) Phylogenetic engineering at an interface between large and small subunits imparts land-plant kinetic properties to algal Rubisco. Proc Natl Acad Sci USA 102:17225-17230