CC-4851 rbcL-L290F/rbcS2-N54S pf2 mt- (R116-1B revertant of 68-4PP) (pf2 progeny used for genetic analysis)
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
R116-1B was recovered as a photosynthesis-competent revertant after methyl-methanesulfonate mutagenesis of rbcL-L290F mt+ (68-4PP) (Hong and Spreitzer 1997). It results from a mutation in rbcS2 that causes an N54S substitution (AAC-AGC) in the Rubisco small subunit, which increases the CO2/O2 specificity of the original mutant enzyme (Du et al. 2000; Genkov et al. 2006). Gene-centromere mapping indicated that rbcS2 is 20 map units from its centromere (Du et al. 2000). This strain was recovered from a cross between the original revertant R116-1B mt+ and pf2 mt-. It has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Du YC, Hong S, Spreitzer RJ (2000) RbcS suppressors enhance the CO2/O2 specificity and thermal stability of rbcL-mutant ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 97:14206-14211
Genkov T, Du YC, Spreitzer RJ (2006) Small-subunit cysteine-65 substitutions can suppress or induce alterations in the large-subunit catalytic efficiency and holoenzyme thermal stability of ribulose-1,5-bisphosphate carboxylase/oxygenase. Arch Biochem Biophys 451:167-174
Hong S, Spreitzer RJ (1997) Complementing substitutions at the bottom of the barrel influence catalysis and stability of ribulose-bisphosphate carboxylase/oxygenase. J Biol Chem 272:11114-11117