CC-4855 rbcS2-C65S mt- (S88-5A suppressor of 68-4PP)
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
Using standard methods of directed mutagenesis and nuclear-gene transformation of rbcS∆-T60-3 mt- (CC-4690), a C65S substitution (TGC-AGC) was created in the Rubisco small subunit (Genkov et al. 2006). This is the small-subunit intergenic-suppressor substitution that complements the large-subunit L290F substitution in photosynthesis-competent revertant R88-5A (rbcL-L290F/rbcS2-C65S). The small-subunit C65S substitution alone improves the thermal stability of wild-type Rubisco (Genkov et al. 2006). This strain has been maintained with acetate medium in darkness since its creation.
Genkov T, Du YC, Spreitzer RJ (2006) Small-subunit cysteine-65 substitutions can suppress or induce alterations in the large-subunit catalytic efficiency and holoenzyme thermal stability of ribulose-1,5-bisphosphate carboxylase/oxygenase. Arch Biochem Biophys 451:167-174