* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-4856 rbcS2-C65A mt-
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
Using standard methods of directed mutagenesis and nuclear-gene transformation of rbcS∆-T60-3 mt- (CC-4690), a C65A substitution (TGC-GCG) was created in the Rubisco small subunit (Genkov et al. 2006). This mutant was created to investigate rbcS suppression of an rbcL mutation. See also R88-5A (rbcL-L290F/rbcS2-C65S). This strain has been maintained with acetate medium in darkness since its creation.
Genkov T, Du YC, Spreitzer RJ (2006) Small-subunit cysteine-65 substitutions can suppress or induce alterations in the large-subunit catalytic efficiency and holoenzyme thermal stability of ribulose-1,5-bisphosphate carboxylase/oxygenase. Arch Biochem Biophys 451:167-174