CC-4857 rbcS2-C65P mt-
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
Using standard methods of directed mutagenesis and nuclear-gene transformation of rbcS∆-T60-3 mt- (CC-4690), a C65P substitution (TGC-CCG) was created in the Rubisco small subunit (Genkov et al. 2006). This mutant was created to investigate rbcS suppression of an rbcL mutation. See also R88-5A (rbcL-L290F/rbcS2-C65S). The C65P substitution in the Rubisco small subunit causes a decrease in CO2/O2 specificity but improves holoenzyme thermal stability (Genkov et al. 2006). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Genkov T, Du YC, Spreitzer RJ (2006) Small-subunit cysteine-65 substitutions can suppress or induce alterations in the large-subunit catalytic efficiency and holoenzyme thermal stability of ribulose-1,5-bisphosphate carboxylase/oxygenase. Arch Biochem Biophys 451:167-174