CC-4858 rbcL-L290F/rbcS∆-CAL005.01.13 mt+ (non-pf2 progeny used for genetic analysis)
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
Phenotype: requires acetate, sensitive to light
This strain was recovered from a cross between rbcL-L290F mt+ (CC-4833) and rbcS∆-CAL005.01.13 pf2 mt- (CC-4693) (Genkov et al. 2006). It was used to investigate Rubisco rbcS2 small-subunit substitutions that complement an rbcL large-subunit substitution in the absence of the rbcS1 gene (Du et al. 2000; Genkov et al. 2006). The strain has been maintained with acetate medium in darkness since its recovery.
Du YC, Hong S, Spreitzer RJ (2000) RbcS suppressors enhance the CO2/O2 specificity and thermal stability of rbcL-mutant ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 97:14206-14211
Genkov T, Du YC, Spreitzer RJ (2006) Small-subunit cysteine-65 substitutions can suppress or induce alterations in the large-subunit catalytic efficiency and holoenzyme thermal stability of ribulose-1,5-bisphosphate carboxylase/oxygenase. Arch Biochem Biophys 451:167-174