CC-4860 rbcL-L290F/rbcS2-WT mt+ (L290F/∆SS1)
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
Phenotype: requires acetate at 35 °C, temperature-conditional
This strain was created by transforming rbcL-L290F/rbcS∆-CAL005.01.13 mt+ with the rbcS2 small-subunit gene (Genkov et al. 2006). Like the original rbcL-L290F mutant (Chen et al. 1988), it can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. The mutant was created for comparison with rbcS suppressors of rbcL-L290F (Genkov et al. 2006). It has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Chen Z, Chastain CJ, Al-Abed SR, Chollet R, Spreitzer RJ (1988) Reduced CO2/O2 specificity of ribulose-1,5-bisphosphate carboxylase/oxygenase in a temperature-sensitive chloroplast mutant of Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 85:4696-4699
Genkov T, Du YC, Spreitzer RJ 2006) Small-subunit cysteine-65 substitutions can suppress or induce alterations in the large-subunit catalytic efficiency and holoenzyme thermal stability of ribulose-1,5-bisphosphate carboxylase/oxygenase. Arch Biochem Biophys 451:167-174