CC-4863 rbcL-L290F/rbcS2-C65S mt+ (L290F/C65S/∆SS1)
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
Plasmid pNESS2-C65S was used to transform rbcL-L290F/rbcS∆-CAL005.01.13 mt+ (Genkov et al. 2006). This strain is like revertant R88-5A (Genkov et al. 2006), but it lacks the wild-type rbcS1 gene. The small-subunit C65S substitution increases the CO2/O2 specificity of the original large-subunit L290F mutant enzyme (Du et al. 2000; Genkov et al. 2006). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Du YC, Hong S, Spreitzer RJ (2000) RbcS suppressors enhance the CO2/O2 specificity and thermal stability of rbcL-mutant ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 97:14206-14211
Genkov T, Du YC, Spreitzer RJ (2006) Small-subunit cysteine-65 substitutions can suppress or induce alterations in the large-subunit catalytic efficiency and holoenzyme thermal stability of ribulose-1,5-bisphosphate carboxylase/oxygenase. Arch Biochem Biophys 451:167-174