CC-4866 rbcL-L290F/rbcS1-L66G mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
By employing standard methods (Genkov et al. 2006), Todor Genkov in Spreitzer’s group used plasmid pSS1-L66G to transform rbcL-L290F/rbcS∆-CAL005.01.13 mt+. The small-subunit L66G substitution suppresses the temperature-conditional phenotype of rbcL-L290F (68-4PP), and increases the CO2/O2 specificity and thermal stability of the original large-subunit L290F mutant enzyme (Genkov and Spreitzer, unpublished). Other small-subunit suppressor substitutions include N54S, A57V, and C65S (Du et al. 2000; Genkov et al. 2006). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Du YC, Hong S, Spreitzer RJ (2000) RbcS suppressors enhance the CO2/O2 specificity and thermal stability of rbcL-mutant ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 97:14206-14211
Genkov T, Du YC, Spreitzer RJ (2006) Small-subunit cysteine-65 substitutions can suppress or induce alterations in the large-subunit catalytic efficiency and holoenzyme thermal stability of ribulose-1,5-bisphosphate carboxylase/oxygenase. Arch Biochem Biophys 451:167-174