CC-4887 wild-type 2137 mt+ (cloned)

$30.00

From Robert J. Spreitzer, University of Nebraska, August 2014

Wild-type 2137 mt+ was recovered from a cross between 21gr mt+ (from Sager) and 137c mt- (from Levine) (Spreitzer 1980; Spreitzer and Mets 1981). Wild-type 21gr mt+ grew in clumps in minimal medium, was green in the dark, and was positively phototactic. Wild-type 137c mt- was yellow in the dark (y1) and negatively phototactic. Wild-type 2137 mt+ was chosen as having good-mating, non-clumping, green-in-the-dark, and negatively-phototactic phenotypes (Spreitzer 1980). The original 2137 mt+ strain has been cloned four times since 1980 to retain its original phenotype. This is the strain used as wild type in Spreitzer’s group (Spreitzer 1998). It has been continuously maintained with acetate medium in darkness.

Spreitzer RJ (1980) A uniparental mutant of Chlamydomonas renhardii with altered ribulose-1,5-bisphosphate carboxylase. Ph. D. thesis, Case Western Reserve University

Spreitzer RJ (1998) The Molecular Biology of Chloroplasts and Mitochondria in Chlamydomonas. Genetic engineering of Rubisco 515-527

Spreitzer RJ, Mets L (1981) Photosynthesis-deficient mutants of Chlamydomonas reinhardtii with associated light-sensitive phenotypes. Plant Physiol 67:565-569