CC-4902 11-4D mt+ (pst-u-1-11-4D) [formerly CC-2054]
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
Phenotype: requires acetate, sensitive to light
Following 5-fluorodeoxyuridine treatment and ethyl-methanesulfonate mutagenesis of wild-type 2137 mt+ cells, colonies were screened for light-sensitive, acetate-requiring phenotypes (Spreitzer and Mets 1981). Mutant 11-4D displayed uniparental inheritance, and lacks photosystem II activity (Spreitzer and Mets 1981). Recombination tests showed that 11-4D is at the same locus (pst-u-1) as 8-36C, 10-4, 11-1A, 11-3D, and 12-3C (Spreitzer and Ogren 1983a). Mutants 8-36C, 11-1A, and 11-4D were shown to result from deletions in psbA (Bennoun et al 1986). However, the extent of the deletions in 11-1A and 11-4D differs from that in 8-36C and FUD7 (CC-4147) (Bennoun et al 1986). This strain was recovered from a cross between the original 11-4D mt+ and pf2 mt- (from Levine), and has been maintained with acetate medium in darkness since 1980 to prevent selection for revertants and non-light-sensitive suppressors (Spreitzer and Ogren 1983b).
Chromosome: chloroplast
Locus: psbA
Bennoun P, Spierer-Herz M, Erickson J, Girard-Bascou J, Pierre Y, Delosme M, Rochaix JD (1986) Characterization of photosystem II mutants of Chlamydomonas reinhardii lacking the psbA gene. Plant Mol Biol 6:151-160
Spreitzer RJ, Mets L (1981) Photosynthesis-deficient mutants of Chlamydomonas with associated light-sensitive phenotypes. Plant Physiol 67:565-569
Spreitzer RJ, Ogren WL (1983a) Rapid recovery of chloroplast mutations affecting ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 80:6293-6297
Spreitzer RJ, Ogren WL (1983b) Nuclear suppressors of the photosensitivity associated with defective photosynthesis in Chlamydomonas reinhardtii. Plant Physiol 71:35-39