CC-4903 10-3C mt+ (pso-u-1-10-3C) [formerly CC-2046]
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
Phenotype: requires acetate, sensitive to light
Following 5-fluorodeoxyuridine treatment and ethyl-methanesulfonate mutagenesis of wild-type 2137 mt+ cells, colonies were screened for light-sensitive, acetate-requiring phenotypes (Spreitzer and Mets 1981). Mutant 10-3C displayed uniparental inheritance, and PSI-deficient fluorescence-induction kinetics (Spreitzer and Mets 1981). Recombination tests showed that 10-3C is at the same locus (pso-u-1) as 12-7 (Spreitzer and Ogren 1983a). Lee et al. (1996) reported that this PSI-deficient mutant could grow photoautotrophically, but the strain they used (CC-2046) likely reverted and has since been lost from the culture collection. This strain was recovered from a cross between the original 10-3C mt+ and pf2 mt- (from Levine), and has been maintained with acetate medium in darkness since 1980 to prevent selection for revertants and non-light-sensitive suppressors (Spreitzer and Ogren 1983b). Jake DeVries in Spreitzer’s group confirmed the original, acetate-requiring phenotype of this mutant as recently as August 2014.
Chromosome: chloroplast
Lee JW, Tevault CV, Owens TG, Greenbaum E (1996) Oxygenic photoautotrophic growth without photosystem I. Science 273:364-367
Spreitzer RJ, Mets L (1981) Photosynthesis-deficient mutants of Chlamydomonas with associated light-sensitive phenotypes. Plant Physiol 67:565-569
Spreitzer RJ, Ogren WL (1983a) Rapid recovery of chloroplast mutations affecting ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 80:6293-6297
Spreitzer RJ, Ogren WL (1983b) Nuclear suppressors of the photosensitivity associated with defective photosynthesis in Chlamydomonas reinhardtii. Plant Physiol 71:35-39