CC-4905 15-1L mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
Phenotype: requires acetate, sensitive to light
Using established methods of 5-fluorodeoxyuridine treatment and ethyl-methanesulfonate mutagenesis of wild-type 2137 mt+ cells (Spreitzer and Mets 1981), colonies were screened for light-sensitive, acetate-requiring phenotypes. Mutant 15-1L displayed uniparental inheritance, and has an 80% decrease in Rubisco carboxylase activity and a 95% decrease in whole-chain electron transport (Spreitzer, unpublished). These pleiotropic alterations may arise from a defect in chloroplast protein synthesis. This strain has been maintained with acetate medium in darkness since it was recovered in 1982 to prevent selection for revertants and non-light-sensitive suppressors (Spreitzer and Ogren 1983b).
Chromosome: chloroplast
Spreitzer RJ, Mets L (1981) Photosynthesis-deficient mutants of Chlamydomonas with associated light-sensitive phenotypes. Plant Physiol 67:565-569
Spreitzer RJ, Ogren WL (1983b) Nuclear suppressors of the photosensitivity associated with defective photosynthesis in Chlamydomonas reinhardtii. Plant Physiol 71:35-39