CC-4906 67-12E mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
Phenotype: requires acetate at 35 °C, temperature-conditional
The 67-12E chloroplast mutant was isolated by screening for temperature-conditional, acetate-requiring mutants (Spreitzer et al. 1988). It can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. Mutant 67-12E has an 80% decrease in oxygen evolution and chlorophyll when grown at 35 °C (Spreitzer et al. 1988), but the genetic basis for defective photosynthesis is not known. This strain has been maintained with acetate medium in darkness to prevent selection for revertants or suppressors.
Chromosome: chloroplast
Spreitzer RJ, Al-Abed SR, Huether MJ (1988) Temperature-sensitive, photosynthesis-deficient mutants of Chlamydomonas reinhardtii. Plant Physiol 86:773-777