CC-4907 69-7M mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
Phenotype: requires acetate at 35 °C, temperature-conditional
The 69-7M chloroplast mutant was isolated by screening for temperature-conditional, acetate-requiring mutants (Spreitzer et al. 1988). It can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. Mutant 69-7M has 90% decreases in Rubisco carboxylase activity and chlorophyll when grown at 35 °C (Spreitzer et al. 1988). These pleiotropic alterations may arise from a defect in chloroplast protein synthesis. This strain was recovered from a cross between the original 69-7M mt+ and pf2 mt-, and has been maintained with acetate medium in darkness to prevent selection for revertants or suppressors.
Chromosome: chloroplast
Spreitzer RJ, Al-Abed SR, Huether MJ (1988) Temperature-sensitive, photosynthesis-deficient mutants of Chlamydomonas reinhardtii. Plant Physiol 86:773-777