* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-4912 Osmo32 mt-
$30.00
From Karin Komsic-Buchmann, Becker lab, University of Cologne, September 2014
This osmoregulatory mutant cannot survive under osmotic conditions below 140 mosM and should be grown on TAP supplemented with 64.6 mM sucrose and 0.2 g/L arginine. It was created by insertional mutagenesis of CC-3395 using pHyg3 and has a deletion in Chromosome 17 of unknown size, one known insertion flanking site (IFS) at 6,748,893.
Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885-95