CC-4926 rbcS1-10SS-LSRR mt-
$30.00
From Robert J. Spreitzer, University of Nebraska, October 2014
Phenotype: requires elevated CO2 for photosynthetic growth
This mutant was created by Fuqiao Xu in Spreitzer’s group by nuclear transformation of rbcSΔ-T60-3 mt- (CC-4690) (Khrebtukova and Spreitzer 1996) with a plasmid (pSS1-10SS-LSRR) that has the Chlamydomonas Rubisco small-subunit coding region of pSS1-ITP (Genkov et al. 2010) after residue 10 replaced with the codon-optimized sequence of Rhodospirillum rubrum Rubisco (Somerville and Somerville 1984). The mutant grows slowly on minimal medium only when provided with 5% CO2 in air. It expresses only a low level of R. rubrum Rubisco, but more than mutant rbcL∆/rbcS1-LSRR mt-. The strain has been maintained with acetate medium in darkness since its recovery in 2012.
Genkov T, Meyer M, Griffiths H, Spreitzer RJ (2010) Functional hybrid Rubisco enzymes with plant small subunits and algal large subunits: Engineered rbcS cDNA for expression in Chlamydomonas. J Biol Chem 285:19833-19841
Khrebtukova I, Spreitzer RJ (1996) Elimination of the Chlamydomonas gene family that encodes the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 93:13689-13693
Somerville CR, Somerville SC (1984) Cloning and expression of the Rhodospirillum rubrum ribulosebisphosphate carboxylase gene in E. coli. Molec Gen Genet 193:214-219