CC-4927 rbcL∆/rbcS1-SSAT pf2 mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, October 2014
Phenotype: requires acetate, sensitive to light, spectinomycin resistant
This Rubisco rbcL knock-out mutant was created by Todor Genkov in Spreitzer’s group by replacing chloroplast rbcL of rbcS1-SSAT pf2 mt+ (CC-4764) with aadA. Whereas the original SSAT strain expresses functional Rubisco comprised of Chlamydomonas large subunits and Arabidopsis small subunits (Genkov et al. 2010), this mutant can express only Arabidopsis rbcS. The mutant was created to see whether functional Arabidopsis Rubisco could be expressed in Chlamydomonas. It has been cloned to homoplasmicity, and maintained with acetate medium in darkness since its isolation.
Genkov T, Meyer M, Griffiths H, Spreitzer RJ (2010) Functional hybrid Rubisco enzymes with plant small subunits and algal large subunits: Engineered rbcS cDNA for expression in Chlamydomonas. J Biol Chem 285:19833-19841