CC-5031 rbcL∆/rbcS1-ABSO mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, November 2014
Phenotype: requires acetate, sensitive to light, spectinomycin resistant
This Rubisco rbcL knock-out mutant was created by Boon Hoe Lim in Spreitzer’s group by using aadA to replace chloroplast rbcL of rbcL-Penta/rbcS1-ABSO mt+ (CC-4848) (Spreitzer et al. 2005). The mutant lacks Rubisco large subunits, but is capable of expressing the ABSO small subunit, which was previously engineered by replacing the Chlamydomonas betaA-betaB loop with the loop from the small subunit of spinach Rubisco (Karkehabadi et al. 2005; Spreitzer et al. 2005). The mutant was created to investigate large-subunit amino acids that differ between Chlamydomonas and land plants, and that interact with small-subunit amino acids (Du et al. 2003). This strain has been cloned to homoplasmicity, and maintained with acetate medium in darkness since its isolation.
Du YC, Peddi SR, Spreitzer RJ (2003) Assessment of structural and functional divergence far from the large subunit active site of ribulose-1,5-bisphosphate carboxylase/oxygenase. J Biol Chem 278:49401-49405
Karkehabadi S, Peddi SR, Anwaruzzaman M, Taylor TC, Cederlund A, Genkov T, Andersson I, Spreitzer RJ (2005) Chimeric small subunits influence catalysis without causing global conformational changes in the crystal structure of ribulose-1,5-bisphosphate carboxylase/oxygenase. Biochemistry 44:9851-9861
Spreitzer RJ, Peddi SR, Satagopan S (2005) Phylogenetic engineering at an interface between large and small subunits imparts land-plant kinetic properties to algal Rubisco. Proc Natl Acad Sci USA 102:17225-17230