CC-504 ery1b mt+
$30.00
Boynton-Gillham laboratory, Duke University
Phenotype: antibiotic resistant (erythromycin)
This is a good isolate of the ery1b mutation, from a cross of CC-460 to a wild type strain. It is resistant to 200+ micrograms/ml erythromycin on plates. It shows only very slight growth on 50 micrograms/ml clindamycin. This strain may also contain a pf marker; very few swimming cells were observed in cultures.
This strain was used in studies by Robert Schmidt in the Boynton-Gillham laboratory in 1983-1985.
The ery1 mutations were shown by Davidson et al. (1974) to alter a protein of the large subunit of the chloroplast ribosome, which was subsequently identified by Bowers et al. as L4.
Davidson JN, Hanson MR, Bogorad L (1974) An altered chloroplast ribosomal protein in ery-M1 mutants of Chlamydomonas reinhardi. Mol Gen Genet 132:119-129
Schmidt RJ, Myers AM, Gillham NW, Boynton JE (1984) Chloroplast ribosomal proteins of Chlamydomonas synthesized in the cytoplasm are made as precursors. J Cell Biol 98:2011-2018
Bowers AK, Keller JA, Dutcher SK (2003) Molecular markers for rapidly identifying candidate genes in Chlamydomonas reinhardtii. Ery1 and ery2 encode chloroplast ribosomal proteins. Genetics 164:1345-1353