CC-5040 rbcL-C256F/K258R/rbcS1-ABSO mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, November 2014
Using standard methods of directed mutagenesis and chloroplast transformation of rbcL∆/rbcS1-ABSO mt+ (Spreitzer et al. 2005), Boon Hoe Lim in Spreitzer’s group created two substitutions (C256F and K258R) together in the Rubisco large subunit. This mutant was created to investigate the molecular basis for the increased CO2/O2 specificity of mutant rbcL-Penta/rbcS1-ABSO mt+ (CC-4848) (Spreitzer et al. 2005). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Spreitzer RJ, Peddi SR, Satagopan S (2005) Phylogenetic engineering at an interface between large and small subunits imparts land-plant kinetic properties to algal Rubisco. Proc Natl Acad Sci USA 102:17225-17230