* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-5132 hydA1-1 mt+
$30.00
From Sarah D’Adamo, Matthew Posewitz lab, Colorado School of Mines, Golden CO, July 2015
Mutation in HYDA1 (Iron hydrogenase) by insertion of pSP124S carrying Zeo R gene (Zeocin). Crossed 4X with D66 (CC-4425 cw15 nit2-203 mt+) and CC-124.
HYDA1: chromosome_3:8216108..822075
Meuser JE, D'Adamo S, Jinkerson RE, Mus F, Yang W, Ghirardi ML, Seibert M, Grossman AR, Posewitz MC (2012) Genetic disruption of both Chlamydomonas reinhardtii [FeFe]-hydrogenases: Insight into the role of HYDA2 in H₂ production. Biochem Biophys Res Commun. 417:704-9