* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-5158 fap46-1::FAP46-HA rescue mt+
$30.00
From Jason Brown, George Witman lab, University of Massachusetts Medical School, October 2015
This strain was generated by transformation of fap46-1 with the plasmid pFAP46-HA containing wild-type FAP46 genomic sequence with a 3x-HA tag inserted immediately 5’ to the stop codon; this plasmid also contains a phleomycin-resistance cassette (Stevens et al., 1996, Mol. Gen. Genet.).
Brown JM, Dipetrillo CG, Smith EF, Witman GB (2012) A FAP46 mutant provides new insights into the function and assembly of the C1d complex of the ciliary central apparatus. J Cell Sci. 125: 3904-3913