CC-517 ac5 mt-
$30.00
From Ursula Goodenough, Harvard University, May 1978
Phenotype: requires acetate on low salt medium
The ac5 mutant was originally reported to form small, yellowish white colonies and to have altered thylakoid membrane stacking when grown on low salt medium. This strain is marginally scorable on Tris-minimal medium. Elevating the temperature seems to enhance the phenotype. Even so, it’s not the best genetic marker for linkage group VII; pf17 and ac1 are easier to work with.
Ebersold WT (1956) Crossing over in Chlamydomonas reinhardi. Amer J Bot 43:408-410
Goodenough UW and Staehelin LA (1971) Structural differentiation of stacked and unstacked chloroplast membranes. Freeze-etch electron microscopy of wild-type and mutant strains of Chlamydomonas. J Cell Biol 48:594-619
Levine RP and Duram HA (1973) The polypeptides of stacked and unstacked Chlamydomonas
reinhardi chloroplast membranes and their relation to photsystem II activity. Biochim Biophys Acta 325:565-572
Steinback KE and Goodenough UW (1975) Morphological and Photosynthetic Properties of Digitonin-treated Chloroplast Membranes from the Wild-type and ac-5 Strains of Chlamydomonas reinhardi. Plant Physiol. 55:864-8