* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-5237 hydEF1::pSP124s-HYDEF mt+ rescue [isolate #9]
$30.00
From Iftach Yacoby, Tel Aviv University, April 2016
Random insertion of the gemonic part of HydEF to rescue the hydEF1 mutant (CC-5128) using the plasmid pSP124s_bleR_HYDEF_KpnI_ins_ (13118bp). Hydrogen production was validated. This was a repeated rescue using the original plasmid from Posewitz et al.
Posewitz MC, King PW, Smolinski SL, Zhang L, Seibert M, Ghirardi ML (2004) Discovery of two novel radical S-adenosylmethionine proteins required for the assembly of an active [Fe] hydrogenase. J Biol Chem. 279:25711-20