* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-5238 drc3-1 mt+ [D4]
$30.00
From Junya Awata, George Witman lab, University of Massachusetts Medical School, May 2016
The nphp4/drc3 strain, B1179 (nphp4-1, drc3-1, arg7, ARG7, mt+) was first generated by insertional mutagenesis of H11 (arg7, mt+) with the pARG7.8 plasmid and crossed sequentially to the wild-type strains CC-124 and 137c. This drc3 strain was then created by transformation of the crossed B1179 with NPHP4.
Mutant cell lacks first 8 out of 10 exons in DRC3 and has defects in swimming speed and flagellar waveform.
Awata J, Song K, Lin J, King SM, Sanderson MJ, Nicastro D, Witman GB (2015) DRC3 connects the N-DRC to dynein g to regulate flagellar waveform. Mol Biol Cell. 26:2788-2800