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CC-5259 pf28 pf30 tpg2-3

Name: CC-5259 pf28 pf30 tpg2-3
SKU: CC-5259
Availability: InStock

$30.00

From Tomohiro Kubo, George Witman lab, University of Massachusetts Medical School, May 2016

Phenotype: reduced axonemal tubulin polyglutamylation, and lacks outer arm dynein and inner arm dynein f/I1; complete immotile

This mutant was isolated as a strain that suppresses the flagellar shortness of pf28, pf30.

LeDizet M, Piperno G (1995) The light chain p28 associates with a subset of inner dynein arm heavy chains in Chlamydomonas axonemes. Mol Biol Cell. 6:697-711

Freshour J, Yokoyama R, Mitchell DR (2007) Chlamydomonas flagellar outer row dynein assembly protein ODA7 interacts with both outer row and I1 inner row dyneins. J Biol Chem. 282:5404-12

Kubo T, Hirono M, Aikawa T, Kamiya R, Witman GB (2015) Reduced tubulin polyglutamylation suppresses flagellar shortness in Chlamydomonas. Mol Biol Cell. 26:2810-22

Locus:
PF28 / DHC15, PF30 / DHC1, TPG2 / FAP234
Chromosome:
11, 12, 1

CC-5259 pf28 pf30 tpg2-3

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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