* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-5326 pf2-4::DRC4-DK-GFP mt-

Name: CC-5326 pf2-4::DRC4-DK-GFP mt-
SKU: CC-5326
Availability: InStock

$30.00

From Kelly Bower, Porter Lab, University of Minnesota, November 2016

This strain was created when pf2-4 mt- (CC-4483) was rescued with DRC4 with a D to K amino acid change at 198 carboxyl terminally tagged with GFP by transformation with pSI103 and selection on paromomycin.

Lewis WR, Malarkey EB, Tritschler D, Bower R, Pasek RC, Porath JD, Birket SE, Saunier S, Antignac C, Knowles MR, Leigh MW, Zariwala MA, Challa AK, Kesterson RA, Rowe SM, Drummond IA, Parant JM, Hildebrandt F, Porter E, Yoder BK, Berbari NF (2016) Mutation of Growth Arrest Specific 8 Reveals a Role in Motile Cilia Function and Human Disease. PLoS Genet. Jul 29;12(7):e1006220. doi:10.1371/journal.pgen.1006220. eCollection 2016 Jul. PubMed PMID: 27472056; PubMed Central PMCID: PMC4966937.

Locus:
PF2
Chromosome:
11

CC-5326 pf2-4::DRC4-DK-GFP mt-

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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