* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-5365 PAM amiRNA1 #5 mt+

Name: CC-5365 PAM amiRNA1 #5 mt+
SKU: CC-5365
Availability: InStock

$30.00

From Steven King, University of Connecticut Health Center, June 2017

This is an amiRNA strain with reduced levels of the enzyme peptidylglycine alpha-amidating monooxygenase (PAM; Cre03.g152850). This strain exhibits a weak knockdown of PAM expression resulting in a mixture of flagellated and unflagellated cells. For a strong knockdown of PAM expression use PAM amiRNA2 #3 or #8

Kumar D, Blaby-Haas CE, Merchant SS, Mains RE, King SM, Eipper BA (2016) Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity. J Cell Sci 129:943-56

Kumar D, Strenkert D, Patel-King RS, Leonard MT, Merchant SS, Mains RE, King SM, Eipper BA (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 10.7554/eLife.25728

CC-5365 PAM amiRNA1 #5 mt+

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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