* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-5369 CrPAM-ΔCD

Name: CC-5369 CrPAM-ΔCD
SKU: CC-5369
Availability: InStock

$30.00

From Steven King, University of Connecticut Health Center, June 2017

This CLiP library strain contains an insertion in the gene for peptidylglycine alpha-amidating monooxygenase (PAM; Cre03.g152850). The insertion occurs in the region encoding the cytosolic C-terminal domain, and results in deletion of the last 75 residues and incorporation of an 18-residue sequence derived from the insertion cassette. This strain exhibits enhanced PAM enzyme activity.

Kumar D, Blaby-Haas CE, Merchant SS, Mains RE, King SM, Eipper BA (2016) Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity. J Cell Sci 129:943-56

Kumar D, Strenkert D, Patel-King RS, Leonard MT, Merchant SS, Mains RE, King SM, Eipper BA (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 10.7554/eLife.25728

CC-5369 CrPAM-ΔCD

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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