* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-5408 pf18 fla3 KAP-GFP

Name: CC-5408 pf18 fla3 KAP-GFP
SKU: CC-5408
Availability: InStock

$30.00

From Hiroaki Ishikawa, Wallace Marshall Lab, UCSF, March 2018

This mutant was created by crossing pf18 (CC-1036) to fla3 KAP-GFP (CC-4296). This strain has the mutant pf18 and fla3 alleles.

Mueller J, Perrone CA, Bower R, Cole DG, Porter ME (2005) The FLA3 KAP subunit is required for localization of kinesin-2 to the site of flagellar assembly and processive anterograde intraflagellar transport. Mol Biol Cell 16:1341–1354

Engel BD, Lechtreck KF, Sakai T, Ikebe M, Witman GB, Marshall WF (2009) Total Internal Reflection Fluorescence (TIRF) Microscopy of Chlamydomonas Flagella. Methods Cell Biol 93:157-177

Engel BD, Ludington WB, Marshall WF (2009) Intraflagellar transport particle size scales inversely with flagellar length: revisiting the balance-point length control model. J Cell Biol 187:81–89

Locus:
PF18, FLA3
Chromosome:
02, 10

CC-5408 pf18 fla3 KAP-GFP

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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