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CC-5416 met1(UVM)-47A mt-

Name: CC-5416 met1(UVM)-47A mt-
SKU: CC-5416
Availability: InStock

$30.00

From Takeshi Ohama, Kochi University of Technology-Japan, September 2018

This strain is good for overexpression of various transgenes. This is probably due to the relieved transcriptional repression.

It was created by transforming CC-4533 with plasmid pMJ013b and paromomycin resistance was used for the selection of transformants (Zhang et al., 2014), resulting in met-1.

The ble-GFP construct was then introduced to met-1 and UV-mutagenesis was carried out to obtain met1(UVM)-47A. This strain is resistant to paromomycin and Zeocin.

Kurniasih SD, Yamasaki T, Kong F, Okada S, Widyaningrum D, Ohama T (2016) UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylation-dependent and independent silencing systems. Plant Mol Biol. 92:629-641

CC-5416 met1(UVM)-47A mt-

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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