CC-548 ac115 mt-
$30.00
From Ursula Goodenough, Harvard University, May 1978
Phenotype: requires acetate
The ac115 and ac115a mutations were UV-induced in Levine’s laboratory.
Kuchka et al. determined that the AC115 gene product specifically promotes translation of the mRNA from the chloroplast psbD gene, encoding the photosystem II D2 protein. In ac115 mutants other proteins of the PS II complex are synthesized but cannot assemble into a stable complex, and are post-translationally degraded.
However, there is a problem: the gene sequenced by Kuchka and collaborators (accessions AF045466 and AF045467; Rattanachaikunsopon et al., 1999) turns out to be on chromosome 8 in the JGI v4 genome sequence, whereas the mutation was mapped to linkage group (chromosome) 1. This conflict has not yet been resolved.
Kuchka MR, Mayfield SP, Rochaix JD (1988) Nuclear mutations specifically affect the synthesis and/or degradation of the chloroplast-encoded D2 polypeptide of photosystem II in Chlamydomonas reinhardtii. EMBO J 7:319-24
Wu HY, Kuchka MR (1995) A nuclear suppressor overcomes defects in the synthesis of the chloroplast psbD gene product caused by mutations in two distinct nuclear genes of Chlamydomonas. Curr Genet 27:263-9
Rattanachaikunsopon P, Rosch C, Kuchka MR (1999) Cloning and characterization of the nuclear AC115 gene of Chlamydomonas reinhardtii. Plant Mol Biol 39:1-10