CC-549 ac141 mt+
$30.00
From Ursula Goodenough, Harvard University, May 1978
Phenotype: requires acetate
The ac141 mutation was UV-induced in Levine’s laboratory. It is easy to score in crosses, and mutant clones form small colonies on tetrad plates.
This mutant was originally reported to be deficient in cytochrome b559 and quinone [Q] activities and had altered thylakoid membranes, with more single discs than wild type strains. Later reports grouped it with other mutants with pleiotropic deficiencies in PS II polypeptides.
A suppressor mutation that restored photosynthetic competence appeared spontaneously in the original ac141 stock and was mapped to linkage group XI by Cosbey et al.,. This mutation also suppressed arg1, ac80a, and ac115, but not arg2, ac31a, ac14k, ac28, ac20, or ac21 The stock containing the original suppressor mutation appears to have been lost.
Levine RP and Volkmann D (1961) Mutants with impaired photosynthesis in Chlamydomonas reinhardi. Biochem Biophys Res Commun. 6:264-269
Hastings PJ, Levine EE, Cosbey E, Hudock MO, Gillham NW, Surzycki SJ, Loppes R, Levine RP (1965) The Linkage groups of Chlamydomonas reinhardi. Microb Genet Bull 23:20-21
Levine RP, Gorman DS (1966) Photosynthetic electron transport chain of Chlamydomonas reinhardi. 3. Light-induced absorbance changes in chloroplast fragments of the wild type and mutant strains. Plant Physiol 41:1293-300
Teichler-Zallen D, Levine RP (1967) An Electron Spin Resonance Study of Manganese in Wild-Type and Mutant Strains of Chlamydomonas reinhardi. Plant Physiol 42:1643-7
Lavorel J, Levine RP (1968) Fluorescence Properties of Wild-Type Chlamydomonas reinhardi and Three Mutant Strains Having Impaired Photosynthesis. Plant Physiol 43:1049-55
Goodenough UW, Levine RP (1969) Chloroplast Ultrastructure in Mutant Strains of Chlamydomonas reinhardi Lacking Components of the Photosynthetic Apparatus. Plant Physiol 44:990-1000
Chua NH, Levine RP (1969) The photosynthetic electron transport chain of Chlamydomonas reinhardi. 8. The 520 nm light-induced absorbance change in the wild-type and mutant strains. Plant Physiol 44:1-6