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CC-5519 dhc1b (temperature sensitive)

Name: CC-5519 dhc1b (temperature sensitive)
SKU: CC-5519
Availability: InStock

$30.00

From Nathan McNeill, George Witman lab, University of Massachusetts Medical School, April 2019

Permissive temperature: 18C – flagella are variable but have average length of one half of wild type.
Non-permissive temperature: 32C – flagella shorten rapidly, average less than half of original length.

Aternate name is dhc1b-2, so as to distinguish it from dhc1b-3. dhc1b-ts and dhc1b-2 are aliases; Lechtreck et al 2009 and Witman 2012 used the former; Lechtreck et al. 2013 used the latter.

Lechtreck KF, Johnson EC, Sakai T, Cochran D, Ballif BA, Rush J, Pazour GJ, Ikebe M, Witman GB (2009) The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella. J Cell Biol. 187:1117-32

Lechtreck KF, Brown JM, Sampaio JL, Craft JM, Shevchenko A, Evans JE, Witman GB (2013) Cycling of the signaling protein phospholipase D through cilia requires the BBSome only for the export phase. J Cell Biol. 201:249-61

Witman, G. B. 2012. Dynein and intraflagellar transport. In: Dyneins: Structure, Biology and Disease (ed. S. M. King). Elsevier, New York, NY. pp. 395-421

CC-5519 dhc1b (temperature sensitive)

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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